Which statement is true about HbA1c testing methods based on the provided notes?

• A. Affinity chromatography is the preferred method for HbA1c measurement.
• B. HPLC is never used for HbA1c testing.
• C. Immunoassays are the only method used for HbA1c.
• D. Capillary electrophoresis is the standard method for HbA1c.

Answer: (A)

Affinity chromatography is designed to selectively capture the glycated form of hemoglobin by using boronic acid groups that form reversible bonds with the glucose moieties on HbA1c. This direct capture of HbA1c provides robust specificity and straightforward sample handling, making it practical for routine clinical use and less susceptible to certain interferences that can affect other methods. That combination of reliable selectivity, ease of use, and suitability for automation explains why it’s described as the preferred approach in the notes.

HPLC is widely used and often serves as a reference method, but it isn’t stated as the preferred method here. Immunoassays are common and convenient but can vary in calibration and potential cross-reactivity. Capillary electrophoresis is another viable option for separating Hb fractions, but it isn’t designated as the standard in these notes.