Which of the following is NOT listed as one of the two most common interferences in hexokinase glucose measurement?

• A. Gross Hemolysis
• B. Increased Bilirubin
• C. Lipemia
• D. Oxygen interference

Answer: (C)

Hexokinase glucose measurement relies on a spectrophotometric readout at 340 nm from NADH produced in the coupled enzymatic reactions. Substances that alter light absorption or cause turbidity can skew the result. The two most commonly noted interferences are gross hemolysis, which releases hemoglobin and changes absorbance, and elevated bilirubin (icterus), which also affects the optical signal. Lipemia, while it can cause turbidity, is not typically listed among the top two interferences for this method, so it is the correct choice for NOT being among the two shared interferences. Oxygen interference is not usually highlighted as a major issue for this specific assay either, but lipemia stands out as the one not among the two commonly reported interferences.