Which MS approach uses isotope dilution to quantify nonprotein nitrogens?

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Multiple Choice

Which MS approach uses isotope dilution to quantify nonprotein nitrogens?

Explanation:
Isotope dilution mass spectrometry relies on adding a precisely known amount of an isotopically labeled version of the target compound as an internal standard. The labeled and unlabeled molecules are chemically identical, so they behave the same during sample preparation and ionization. The mass spectrometer can distinguish them by a small mass difference, and quantification is based on the ratio of their signals. This ratio compensates for losses and matrix effects, giving accurate measurement of nonprotein nitrogens even in complex samples. The other MS approaches are general techniques or separation methods, but they do not inherently describe the quantitative isotope-dilution strategy.

Isotope dilution mass spectrometry relies on adding a precisely known amount of an isotopically labeled version of the target compound as an internal standard. The labeled and unlabeled molecules are chemically identical, so they behave the same during sample preparation and ionization. The mass spectrometer can distinguish them by a small mass difference, and quantification is based on the ratio of their signals. This ratio compensates for losses and matrix effects, giving accurate measurement of nonprotein nitrogens even in complex samples. The other MS approaches are general techniques or separation methods, but they do not inherently describe the quantitative isotope-dilution strategy.

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