Which lipase determination method measures the rate of clearing by turbidity?

• A. Turbidimetric
• B. Cherry Crandall (titrimetric)
• C. Colorimetric glycerol kinase + peroxidase
• D. Enzyme-linked immunoassay

Answer: (A)

The method being tested is the turbidimetric approach. Here, lipase activity is measured by how quickly the oil-in-water emulsion becomes clearer as triglycerides are hydrolyzed. The enzyme breaks down fat droplets, reducing their turbidity, so you track the rate of clearing by measuring the change in turbidity over time—the faster the clearing, the higher the lipase activity.

This is a direct kinetic readout of emulsified lipid hydrolysis, which is why it’s the best fit for measuring the rate of clearing by turbidity. Other methods measure different things: titrimetry (Cherry Crandall) follows the amount of fatty acids released by titration, colorimetric assays with glycerol kinase and peroxidase detect glycerol produced, and enzyme-linked immunoassay detects the amount of lipase protein rather than its activity or the turbidity change.