Which lipase assay uses colorimetric detection with glycerol kinase and peroxidase?

• A. Colorimetric glycerol kinase + peroxidase
• B. Cherry Crandall titrimetric
• C. Turbidimetric
• D. Immunoassay

Answer: (A)

This question is about how lipase activity is measured by a colorimetric method that uses a glycerol-based readout. Lipase acts on triglycerides to release glycerol, and the amount of glycerol produced is proportional to the lipase activity. In the glycerol kinase–peroxidase colorimetric assay, glycerol is first converted by glycerol kinase and ATP to glycerol-3-phosphate. Glycerol-3-phosphate is then oxidized, producing hydrogen peroxide, which peroxidase uses to oxidize a chromogenic substrate. The resulting color intensity is measured spectrophotometrically and directly reflects the amount of glycerol—and thus the lipase activity. This approach is distinct from titrimetric methods, which rely on volumetric measurements, turbidimetric methods, which track changes in turbidity, or immunoassays, which use antibodies.