Which LDL-C determination method is a direct homogeneous assay that uses detergents?

• A. Beta Quantification
• B. Homogenous Direct Assay
• C. Indirect Calculation
• D. Ultracentrifugation

Answer: (B)

Direct homogeneous LDL-C assays quantify LDL-cholesterol in one step without first separating the lipoprotein fractions. Detergents are included to solubilize lipids and suppress interference from other lipoproteins, allowing the LDL-C signal to be read directly in a single phase. This combination—no separation, and a detergent-enhanced direct measurement—defines the method described. Other options rely on separation techniques (Beta Quantification and ultracentrifugation) or on calculation from other lipid values (indirect calculation), rather than a single-step direct measurement.