Which LD isoenzyme remains active after heat denaturation?

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Multiple Choice

Which LD isoenzyme remains active after heat denaturation?

Explanation:
Lactate dehydrogenase has several isoenzymes that differ in how stable they are when heated. These enzymes are tetramers made from heart (H) and muscle (M) subunits. Isoenzymes richer in H subunits tend to be more heat-stable, while those with more M subunits denature more easily. When you heat the serum to a typical denaturing temperature, most isoenzymes lose activity, but the heart-type isoenzyme, LDH-1 (H4), stays active. Therefore, the isoenzyme that remains active after heat denaturation is LDH-1, with the others being inactivated.

Lactate dehydrogenase has several isoenzymes that differ in how stable they are when heated. These enzymes are tetramers made from heart (H) and muscle (M) subunits. Isoenzymes richer in H subunits tend to be more heat-stable, while those with more M subunits denature more easily. When you heat the serum to a typical denaturing temperature, most isoenzymes lose activity, but the heart-type isoenzyme, LDH-1 (H4), stays active. Therefore, the isoenzyme that remains active after heat denaturation is LDH-1, with the others being inactivated.

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