Which instrument is used for the colorimetric enzymatic cholesterol assay?

• A. Centrifuge
• B. Spectrophotometer
• C. Mass Spectrometer
• D. Polarimeter

Answer: (B)

Colorimetric enzymatic cholesterol assays rely on producing a colored product whose intensity reflects the cholesterol level. To quantify that color, you need an instrument that measures how much light the sample absorbs at a specific wavelength, which is exactly what a spectrophotometer does. It shines light through the sample and records the absorbance, allowing you to correlate color intensity with concentration via a standard curve. The other instruments don’t measure color changes: a centrifuge separates components, a mass spectrometer analyzes molecular mass, and a polarimeter assesses the rotation of polarized light.