Which enzyme is common to both enzymatic triglyceride measurement methods?

• A. Lipase
• B. Glycerokinase
• C. Glycerophosphate oxidase
• D. Peroxidase

Answer: (A)

Two enzymatic triglyceride measurement methods both hinge on freeing glycerol from the triglyceride molecule. This initial release is accomplished by lipase, and it is essential because the downstream detection steps measure glycerol-derived products, not the intact triglyceride. After glycerol is liberated, pathways often involve glycerol kinase converting it to glycerol-3-phosphate, then glycerol-3-phosphate oxidase producing hydrogen peroxide, which peroxidase uses to generate the color signal. While the exact downstream enzymes can vary between methods, the lipase-driven release of glycerol is the common, indispensable step across these enzymatic approaches. The other enzymes listed participate in the detection cascade, but they are not universal requirements in the initial liberation step.