Which compound is the other antiglycolytic agent besides sodium fluoride?

• A. Sodium fluoride
• B. Iodoacetate
• C. Ethanol
• D. Glucose

Answer: (B)

The concept being tested is how glycolysis can be blocked to prevent glucose from being consumed in a sample. Sodium fluoride is one well-known antiglycolytic agent because it inhibits enolase late in glycolysis, helping to preserve glucose levels in blood specimens.

The other antiglycolytic agent commonly recognized is iodoacetate. It inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme early in the glycolytic pathway. By blocking GAPDH, glycolysis is halted and glucose remains unutilized. This makes iodoacetate a classic complementary inhibitor to fluoride for stopping glycolysis in experimental settings.

Ethanol is not a standard antiglycolytic preservative in clinical practice, and glucose is the substrate that glycolysis uses, not an inhibitor.