Which ALP isoenzyme is most resistant to heating at 65°C for 30 minutes?

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Multiple Choice

Which ALP isoenzyme is most resistant to heating at 65°C for 30 minutes?

Explanation:
Heat stability varies among ALP isoenzymes because of differences in their protein structure and glycosylation. Placental alkaline phosphatase is more structurally stable, so it resists denaturation at higher temperatures. When serum is heated to 65°C for 30 minutes, the other isoenzymes—hepatic, bone, and intestinal—tend to unfold and lose activity, while placental ALP remains more intact and active. This makes placental ALP the most resistant to that heating condition.

Heat stability varies among ALP isoenzymes because of differences in their protein structure and glycosylation. Placental alkaline phosphatase is more structurally stable, so it resists denaturation at higher temperatures. When serum is heated to 65°C for 30 minutes, the other isoenzymes—hepatic, bone, and intestinal—tend to unfold and lose activity, while placental ALP remains more intact and active. This makes placental ALP the most resistant to that heating condition.

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