When a standard curve has good linearity, what does y represent?

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Multiple Choice

When a standard curve has good linearity, what does y represent?

Explanation:
In a standard curve used for quantitation, the measured signal on the vertical axis is the absorbance. If the curve has good linearity, absorbance increases proportionally with concentration, following A = εlc. So the y value represents absorbance. The concentration is plotted on the horizontal axis, while the slope and intercept are properties of the line (calibration factor and background offset), not the measured response.

In a standard curve used for quantitation, the measured signal on the vertical axis is the absorbance. If the curve has good linearity, absorbance increases proportionally with concentration, following A = εlc. So the y value represents absorbance. The concentration is plotted on the horizontal axis, while the slope and intercept are properties of the line (calibration factor and background offset), not the measured response.

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