What is the electrophoresis order of ALP isoenzymes from most anodic to least anodic?

• A. L B P I Regan
• B. Regan I P B L
• C. L Regan B I P
• D. I L P Regan B

Answer: (A)

Electrophoretic separation depends on net charge at the working pH: more negative charge means greater migration toward the anode (more anodic). ALP isoenzymes differ in glycosylation and sialic acid content, which changes their isoelectric point and how far they move in a given buffer.

Among the ALP isoforms, the liver form carries the greatest negative charge under standard conditions, so it migrates farthest toward the anode. Next is the bone isoenzyme, then the placental, followed by the intestinal form. The Regan isoenzyme has the least negative charge, so it moves the least toward the anode.

Thus the order from most anodic to least anodic is: liver, bone, placental, intestinal, Regan.