What is the color observed in the standard alkaline phosphatase colorimetric method?

Prepare for the Clinical Chemistry Numericals Test. Study with comprehensive questions, each with detailed explanations. Boost your confidence and excel on your exam!

Multiple Choice

What is the color observed in the standard alkaline phosphatase colorimetric method?

Explanation:
In this colorimetric assay, the enzyme cleaves a substrate called p-nitrophenyl phosphate to release p-nitrophenol. In the alkaline reaction mix, p-nitrophenol becomes deprotonated to p-nitrophenolate, which appears yellow. The more enzyme activity, the more yellow pigment you see, typically measured by absorbance around 405 nm. So the observed color is yellow.

In this colorimetric assay, the enzyme cleaves a substrate called p-nitrophenyl phosphate to release p-nitrophenol. In the alkaline reaction mix, p-nitrophenol becomes deprotonated to p-nitrophenolate, which appears yellow. The more enzyme activity, the more yellow pigment you see, typically measured by absorbance around 405 nm. So the observed color is yellow.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy