VLDL is faster than LDL in electrophoresis because VLDL is more negatively charged. True or false?

• A. True
• B. False
• C. Not determinable
• D. Both are equally charged

Answer: (A)

In electrophoresis, how fast a particle moves is mainly determined by its net charge relative to its size—the more negative the surface charge, the faster it will migrate toward the anode. VLDL carries more surface negative charge than LDL because of its phospholipid-rich surface and apolipoprotein makeup, giving it a higher charge-to-mass ratio. Even though VLDL particles are larger, this greater negative charge drives quicker movement, placing it ahead of LDL in the gel (the pre-beta region versus the beta region). So the statement is true.