In the Porter-Silber test for hydroxycorticosteroids, what color is produced when DNPH is used?

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Multiple Choice

In the Porter-Silber test for hydroxycorticosteroids, what color is produced when DNPH is used?

Explanation:
Porter-Silber uses 2,4-dinitrophenylhydrazine to react with the carbonyl group in hydroxycorticosteroids, forming a yellow hydrazone derivative. The yellow color indicates a positive result because the hydrazone chromophore absorbs light in the blue-green region, giving that yellow hue. Purple, pink, or green do not appear for this reaction under standard conditions.

Porter-Silber uses 2,4-dinitrophenylhydrazine to react with the carbonyl group in hydroxycorticosteroids, forming a yellow hydrazone derivative. The yellow color indicates a positive result because the hydrazone chromophore absorbs light in the blue-green region, giving that yellow hue. Purple, pink, or green do not appear for this reaction under standard conditions.

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