In the enzymatic determination of urea, what is produced when urea reacts with urease?

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Multiple Choice

In the enzymatic determination of urea, what is produced when urea reacts with urease?

Explanation:
Urease catalyzes the hydrolysis of urea to carbon dioxide and ammonia. In water, the ammonia quickly exists as ammonium (NH4+) at physiological pH, so the product you detect in enzymatic urea assays is ammonium. This is the form measured because the assay often converts the produced ammonium into a detectable colorimetric signal. Nitrite or nitrate are not produced by this reaction; they come from different nitrogen-oxidation processes, not from urease activity.

Urease catalyzes the hydrolysis of urea to carbon dioxide and ammonia. In water, the ammonia quickly exists as ammonium (NH4+) at physiological pH, so the product you detect in enzymatic urea assays is ammonium. This is the form measured because the assay often converts the produced ammonium into a detectable colorimetric signal. Nitrite or nitrate are not produced by this reaction; they come from different nitrogen-oxidation processes, not from urease activity.

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